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华理研制八联微型反应器 助推工业菌种快速进化

转自微信公众号:BIOB资讯

u2电竞WEISHENGWUJUNZHONGSHIFAJIAOGONGYEDEJICHU,XINGNENGYOULIANGDEJUNZHONGSHISHIXIANSHENGWUZHIPINJINGJIGAOXIAOSHENGCHANDEGUANJIAN。GAOTONGLIANGSHAIXUANJISHUYIZIDONGHUACAOZUOXITONGJINXINGSHIYANCAOZUO,YIKAOLINGMINKUAISUDEJIANCEYIQICAIJIXINHAO,TONGSHIJIANCEDAPILIANGDEYANGPIN。YINCI,CAIYONGGAOTONGLIANGSHAIXUANJISHUJINXINGJUNZHONGXUANYUNENGGOUGENGGAOXIAODIHUODEYOULIANGSHENGCHANJUNZHU,TIGAOSHENGWUZHIZAOQIYEDEGUOJIJINGZHENGLI。

高通量培养新装备:带有先进传感器的八联微型生物反应器

在菌种选育中,培养过程的微型化是提高筛选通量的重要手段,通过缩小培养装置不仅可以减少人工和材料成本,还可以增加实验的平行性。理想的微培养装置应该是能够准确模仿大规模培养过程,真实地反应微生物的生理代谢和生产能力。在高通量筛选模型中,传统的复筛方式是摇瓶培养,但是其存在数据密度低,培养条件不能调控、过程参数无法在线实时采集等问题,因此在对于发酵罐菌株性能验证存在一定的放大局限性。微型生物反应器保留了传统生物反应器的实时监测功能,体积介于摇瓶和微孔板之间,不仅带有搅拌桨与进气管,还配有光化学pH和DO传感器,能够对大型生物反应器的特征进行完全模拟,并同时对多个实验进行平行运转,是进行细胞株筛选、培养基优化和工艺优化的重要设备。该论文将自行研制开发的八联平行微反应器(图1)引入到高通量筛选模型中,用于替代复筛过程的摇瓶(图2),从而在保证筛选通量的基础上,提高菌株性能验证的可放大性。

图1.  八联微型生物反应器系统

TU2  JUNZHONGGAOTONGLIANGSHAIXUANLIUCHENGTU

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WENXIANLAIYUAN:Xiwei Tian, Gang Zhou, Weifei Wang, Ming Zhang, Haifeng Hang, Ali Mohsin, Ju Chu, Yingping Zhuang, Siliang Zhang. Application of 8-parallel micro-bioreactor system with non-invasive optical pH and DO biosensor in high-throughput screening of l-lactic acid producing strain. Bioresources and Bioprocessing, 2018, 5: 20.

u2电竞BIOBQIKANQITALUNWENLIANJIE:http://link.springer.com/journal/40643

u2电竞2017, Volume 4 SUOYOULUNWENLIANJIE(GONG56PIAN):

u2电竞http://link.springer.com/journal/40643/4/1/page/1

u2电竞2016, Volume 3 SUOYOULUNWENLIANJIE(GONG53PIAN):

http://link.springer.com/journal/40643/3/1/page/1

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